Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

* * *

Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Campylobacter

Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Coxiella burnetii

Cryptosporidium

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Helicobacter

Hepatitis E

Histoplasma

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leptospira

Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia

Pseudorabies

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Ferret respiratory enteric coronavirus PCR test
wildlife and zoo assay data sheet

Ferret respiratory enteric coronavirus (FRECV)

Test code: S0185
- Ultrasensitive qualitative detection of ferret respiratory enteric coronavirus by reverse transcription coupled real time PCR

Coronaviruses (CoVs) are enveloped RNA viruses containing a positive-sense single-stranded RNA genome. These viruses can cause acute and chronic respiratory, enteric, and central nervous system disease in a variety of animal species. Recently, a novel ferret enteric coronavirus, FRECV, was identified in domesticated ferrets (Mustela putorius). Ferret enteric coronavirus causes an enteric disease called epizootic catarrhal enteritis (ECE). Infected ferrets develop foul-smelling green diarrhea with high mucus content, lethargy, anorexia, and vomiting.

More recently, a new systemic coronavirus-associated disease closely resembling the granulomatous or dry form of feline infectious peritonitis (FIP) was reported in ferrets of the United States and Europe. This disease is believed to be caused by ferret systemic coronavirus (FRSCV).

Phylogenetic analyses based on predicted amino acid sequences showed that FRECV is genetically most closely related to group 1 coronaviruses. Group 1 coronaviruses include some important causes of enteric disease in domestic animals, including transmissible gastroenteritis virus of swine, feline coronavirus (FCoV), and canine coronavirus. Both the ferret enteric coronavirus (FRECV) and the ferret systemic coronavirus (FRSCV) were recently identified as group 1 coronaviruses.

ECE is a highly contagious diarrheal disease, with outbreaks usually involving 100% of ferrets housed together in a household or breeding facility. Even though the morbidity for ECE often reaches 100%, generally the mortality rate is low (<5%). Young ferrets infected with FRECV usually develop mild symptoms or no symptoms at all, but older ferrets are more severely affected and have higher mortality rates (Murray et al., 2010).

Other methods of detecting FRECV are less specific than PCR due to cross-reactivity with other coronaviruses, so diagnosis of FRECV and ECE is usually done by PCR.

Utilities:            

  • Help confirm the disease causing agent
  • Identify carriers of this virus
  • Screen research materials for the presence of this virus
  • Help ensure that ferret facilities are free of this virus
  • Early prevention of spread of the virus among animals
  • Minimize human exposure to the virus
  • Safety monitoring of biological products that derive from susceptible animals

References:
Murray, J., Kiupel, M. and Maes, R.K. (2010) Ferret coronavirus-associated diseases. Vet. Clin. Exot. Anim. 13:543–560.

Specimen requirements: Fecal swab, or 0.2 ml feces, or respiratory swab, or 0.2 ml fresh or frozen tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

2003-2016 Zoologix, Inc. • Email Zoologix • Phone (818) 717-8880