Ruminating about hoofstock "issues"?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our new Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

* * *

Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Campylobacter

Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Coxiella burnetii

Cryptosporidium

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Helicobacter

Hepatitis E

Histoplasma

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leptospira

Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia

Pseudorabies

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Helicobacter PCR tests for animals
wildlife and zoo assay data sheet

Helicobacter

Test codes:

B0021 - Ultrasensitive qualitative detection of Helicobacter pylori by real time polymerase chain reaction

B0023 - Ultrasensitive qualitative detection of Helicobacter heilmannii by real time polymerase chain reaction

B0097 - Ultrasensitive qualitative detection of Helicobacter mustelae by real time polymerase chain reaction

P0010 - Ultrasensitive Helicobacter species screen by real time polymerase chain reaction.  This screen detects but does not differentiate H. pylori, H. heilmannii, H. bilis, H. hepaticus, H. rappini, H. felis, H. salomonis and other Helicobacter species.

P0011 - Ultrasensitive Helicobacter species identification by real time polymerase chain reaction and Restriction Fragment Length Polymorphism.  This 2-stage assay detects and differentiates H. pylori, H. heilmannii, H. bilis, H. hepaticus, H. rappini, H. felis, H. salomonis and other Helicobacter species.

 

Many species of the genus Helicobacter have been identified in mammals and their pathogenicity varies. Some species can induce significant disease while others appear to merely colonize the gastrointestinal tract.

Helicobacter pylori
Helicobacter pylori is a gram-negative spiral bacterium found in gastric mucosa and associated with active and chronic gastritis. H. pylori can establish a chronic, persistent infection, which may lead to gastric or duodenual ulcers, gastric cancer and gastric lymphomas. Studies have revealed that approximately 50% of the world’s human population is infected with H. pylori.

Biochemically, the bacterium produces catalase, oxidase and urease enzymes. The urease enzyme permits the bacterium to metabolize urea present in the gastric mucosa and establish a microenvironment favorable to the organism. H. pylori is a highly motile organism with multiple unipolar flagella. Both the urease enzyme and the flagella are considered to be important virulence factors.

Diagnosis of Helicobacter pylori infection in humans relies on upper endoscopy or the 13C-urea breath test (see review by Nakamura, 2001). Although the endoscopy procedure permits culture of the bacterium from biopsy specimens (the gold standard for diagnosis), demonstration of urease activity and histological detection of the germ, the procedure is expensive and invasive. The 13C-urea breath test is a well-established, relatively sensitive, specific and noninvasive method. Molecular tests, such as PCR, can also offer precise diagnosis of H. pylori infections. In fact, molecular testing by PCR can complement other diagnostic tests because it can be applied to archival fixed tissue, environmental samples, gastric fluid, oral secretions, and stool samples, in which traditional diagnostic tests do not have sensitivity and perform poorly. Studies have shown than PCR detection of H. pylori in gastric fluid specimens can reach a sensitivity of 96% and a specificity of 100% (Westblom et al., 1993; Yoshida et al., 1999). This capability is especially useful in monitoring active H. pylori infection in primates and other animals, as the breath test is difficult to conduct for these animals.

Helicobacter heilmannii
Helicobacter heilmannii
(previously known as Gastrospirillum hominis) is a 4-10 m long, spiral-shaped, motile bacterium with three to eight coils, a wavelength of about 1 m, up to 14 uni- or bipolar flagella, and no periplasmic filaments. In humans, gastric infection with H. heilmannii is associated with the development of chronic gastritis (found in the stomachs of 0.2 to 4% of patients with gastritis) and low-grade mucosa-associated lymphoid tissue lymphoma in humans. Eradication of H. heilmannii by antibiotic treatment of patients can result in complete remission of MALT lymphoma, indicating a causal relationship between H. heilmannii infection and MALT lymphoma. Unlike H. pylori infections, gastric infections with H. heilmannii or Gastrospirillum-like organisms are not restricted to humans. A broad range of animals, including dogs, cats, pigs, and cattle, are naturally infected, with frequencies ranging from 80% to 100%. It has been suggested that H. heilmannii infection in humans may be a zoonosis and that animals may serve as a reservoir for transmission to humans.

Definitive culture of H. heilmannii has not been achieved to date (Anderson et al., 1996) and diagnosis of H. heilmannii infection is usually made on the basis of its distinct spiral morphology, compared with H. pylori, on silver- stained tissue sections. However, there are a number of large, gastric, spiral organisms such as H. felis, H. salomonis, and H. bizzozeronii which are indistinguishable from H. heilmannii on routine light microscopy, and H. pylori grown in a broth culture can also adopt a morphology identical to that of H. heilmannii (Fawcett et al., 1999). Molecular detection methods, such as PCR, are always required for more definitive identification (Trebesius et al., 2001).

Helicobacter mustelae
The genus Helicobacter contains more than 50 species which have been isolated from a wide range of vertebrate hosts. Among these species, Helicobacter mustelae is especially interesting because it is the only Helicobacter species other than H. pylori that is known to cause gastritis, ulcers and gastric cancer, though these only occur in ferrets and other mustelids.

When ferrets are infected with H. mustelae, the bacteria stimulate gastric epithelial cells to proliferate and it has been suggested that this stimulation results in gastric adenocarcinoma and MALT lymphoma formation. Ulcers are also common in infected ferrets. In clinical research, H. mustelae infection of ferrets is the only natural model of Helicobacter-associated ulcer disease.

It has been suggested that nearly 100% of ferrets may be infected with Helicobacter mustelae by weaning. However, only a small percentage of these ferrets show clinical symptoms. Disease is seen most commonly in ferrets that have been stressed or have other concurrent diseases. Clinical signs of infection include lack of appetite, diarrhea, black tarry stools, vomiting and abdominal pain (as manifested by grinding teeth, hunched posture, and reluctance to move). Signs of nausea such as increased salivation and pawing at the mouth may be seen. With chronic infection, weight loss, weakness, and loss of muscle mass are common symptoms.

Culture detection of H. mustelae is difficult because of its fastidious growth requirements. Molecular detection by polymerase chain reaction (PCR) is rapid, sensitive and specific, and is a good alternative to the traditional approach (Forester et al., 2003.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Helicobacter infection
  • Help ensure that animal facilities are free of Helicobacter
  • Early prevention of spread of these bacteria among a group of animals
  • Minimize personnel exposure to these bacteria
  • Safety monitoring of biological products and vaccines that derive from susceptible animals

References:
Nakamura, R.M. (2001) Laboratory tests for the evaluation of Helicobacter pylori infections. J. Clin. Lab. Analysis 15: 301-307.
Westblom, T.U., Phadmis, S., Yang, P. and Czinn, S.J. (1993) Diagnosis of Helicobacter pylori infection by means of a polymerase chain reaction for gastric juice aspirates. Clin. Infect. Dis. 16: 367-371.
Yoshida, H., Maeda, S. and Ogura, K. (1999) PCR-monitoring of gastric juice obtained with the capsulated string for evaluation of H. pylori infection. Nippon Rhinsho 57: 107-110.
Andersen, L.P., Norgaard, A., Holck, S., Blom, J. and Elsborg, L. (1996) Isolation of a "Helicobacter heilmannii"-like organism from the human stomach. Eur. J. Clin. Microbiol. Infect. Dis. 15:95-96.
Fawcett, P.T., Gibney, K.M. and Vinette, K.M. (1999) Helicobacter pylori can be induced to assume the morphology of Helicobacter heilmannii. J. Clin. Microbiol. 37:1045-1048.
Trebesius, K., Adler, K., Vieth, M., Stolte, M. and Haas, R. (2001) Specific detection and prevalence of Helicobacter heilmannii-like organisms in the human gastric mucosa by fluorescent in situ hybridization and partial 16S ribosomal DNA sequencing. J. Clin. Microbiol. 39:1510-1516.
Forester, N.T., Lumsden, J.S., Parton, K., Cowan, P.E. and O'Toole, P.W. (2003) Detection and isolation of Helicobacter mustelae from stoats in New Zealand. New Zealand Vet. J. 51:142-145.

Specimen requirement: 0.2 ml gastric lavage, or rectal swab, or 0.2 ml feces, or 0.2 ml fresh, frozen or fixed tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodologies:
B0021
:  Qualitative real time PCR
B0023
:  Qualitative real time PCR
B0097
:  Qualitative real time PC
R
P0010:  Qualitative real time PCR
P0011:  Qualitative real time PCR and RFLP

Normal range: Nondetected

2003-2017 Zoologix, Inc. • Email Zoologix • Phone (818) 717-8880