Moving reptiles?  Use our snake and lizard quarantine PCR panel to avoid spreading contagious agents.

Ruminating about hoofstock issues?  Try our ruminant fecal screening PCR panel - tests for most common GI pathogens in wild & domestic ruminants.

Our Rodent Infestation PCR Panel tests for 5 common pathogens found in rodent-contaminated facilities.

In over your head? Try our waterborne pathogens PCR panel - detection of 7 different environmental pathogens by real time PCR.

Something fishy going on in your tanks? Try our Zebrafish screening PCR panel - tests for 6 different pathogen categories from one easy-to-collect sample.

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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

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Aspergillus

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Cryptosporidium varanii (formerly saurophilum)

Delftia acidovorans

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E. coli panel

Edwardsiella

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Enterovirus

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Feline immunodeficiency virus (FIV)

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Japanese encephalitis

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Lizard quarantine panel

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Macropodid (kangaroo) herpesviruses

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Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Ophidiomyces ophiodiicola

Pasteurella multocida

Pentastomid worms

Plasmodium species

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

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Pseudocapillaria tomentosa

Pseudocapillaroides xenopi

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Pseudoterranova worms

Q fever

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Raillietiella orientalis

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Reovirus screen

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Snake fungal disease

Snake quarantine panel

Stenotrophomonas maltophilia

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West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Pasteurella multocida PCR test

wildlife and zoo assay data sheet

Pasteurella multocida NOTE: THIS TEST IS NOT PERFORMED ON SAMPLES TAKEN FROM BOVINES OWNED OR LOCATED IN THE STATE OF CALIFORNIA.

Test code: B0045 - Ultrasensitive qualitative detection of Pasteurella multocida by real time PCR

 

Pasteurella bacteria are small, nonmotile, gram-negative, bipolar-staining bacilli that normally inhabit the nasal, gingival and tonsillar regions of most domestic cats, many dogs and many other animal species. P. multocida is also associated with hemorrhagic septicemia in elephants.

Pasteurella secretes an endotoxin that changes the properties of the pulmonary surfactant. This alters pulmonary mechanics and gas exchange, often resulting in a pneumonia which is slow to resolve. Abscesses or pleuritis may also result from Pasteurella infection.

Pasteurella multocida is the most common respiratory pathogen in the domestic rabbit. Infection with the bacteria can result in rhinitis, conjunctivitis, pneumonia, abscesses, genital tract infections, and septicemia. The bacteria can be transmitted via aerosol or contact with an infected animal, either directly or through fomites. P. multocida initially colonizes the pharynx, then moves to the nasal cavity and surrounding tissue, with the potential to spread to the rest of the body. Colonization of the nasal cavity may take two weeks to occur, at which time clinical signs may or may not appear. Infected rabbits may become carriers without exhibiting clinical signs.

Traditionally, diagnosis of Pasteurella was based on clinical findings, culture and/ or serological testing. Although culture identification methods are definitive, they are time consuming and costly. False-negative culture results are frequently observed due to the fact that P. multocida dies easily during transport to the laboratory or is overgrown by other bacteria (nasal flora and contaminants) in the culture.

Serology can be used for cases when infection is suspected in organs for which cultures are not attainable, or when culturing has yielded no results. However, a seropositive titer to P. multocida merely indicates past exposure to the organism. Because many rabbits and other animals have been exposed to this organism, a diagnosis of pasteurellosis cannot be made based on serologic results alone. Molecular detection by PCR, however, offers a highly sensitive, accurate and timely method for detecting Pasteurella and confirming current infection (Miflin and Blackall, 2001).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Pasteurella
  • Help ensure that rabbit facilities are free of Pasteurella
  • Early prevention of spread of Pasteurella among a facility
  • Minimize personnel exposure to Pasteurella
  • Safety monitoring of biological products that derive from rabbits and other animals

References:
Miflin, J. K. and Blackall, P. J. (2001) Development of a 23 S rRNA-based PCR assay for the identification of Pasteurella multocida. Lett. Appl. Microbiol. 33: 216–221.

Specimen requirements: Nasal swab or 0.2 ml whole blood in EDTA.

Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time polymerase chain reaction

Normal range: Nondetected

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