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Zoologix performs environmental, zoo, wildlife and aquatic PCR tests for...

Aeromonas hydrophila

African swine fever

Aleutian disease

Amphibian panel

Aspergillus

Babesia

Batrachochytrium dendrobatidis

Baylisascaris procyonis

Borna virus

Borrelia burgdorferi

Campylobacter

Canine distemper

Canine parvovirus

Chytrid fungus

Citrobacter freundii

Classical swine fever

Clostridium

Coccidia

Coccidioides

Coronaviruses

Coxiella burnetii

Cryptosporidium

Cryptosporidium serpentis

Delftia acidovorans

E. coli O157:H7

E. coli panel

Edwardsiella

Encephalomyocarditis

Enterobacteraceae

Enterovirus

Epizootic hemorrhagic disease

Feline immunodeficiency virus (FIV)

Feline infectious peritonitis (FIP)

Feline panleukopenia

Ferret respiratory enteric coronavirus

Giardia

Hantavirus

Helicobacter

Hepatitis E

Histoplasma

Japanese encephalitis

Johne's disease

Kangaroo herpesviruses

Klebsiella

Lawsonia intracellularis

Legionella

Leishmania

Leptospira

Listeria monocytogenes

Lyme disease

Macropodid (kangaroo) herpesviruses

Mink enteritis virus

Monkeypox

Mycobacteria in mammals, amphibians and fish

Mycoplasma mustelae

Mycoplasma species

Neospora caninum

Nipah virus

Pasteurella multocida

Porcine cytomegalovirus

Porcine lymphotropic herpesvirus

Porcine parvovirus

Pseudocapillaria tomentosa

Pseudoloma neurophilia

Pseudorabies

Q fever

Rabies

Ranavirus

Reovirus screen

Rickettsia

Rift Valley fever

Rotavirus

Salmonella

Sarcocystis neurona

Stenotrophomonas maltophilia

St. Louis encephalitis

Strep pneumoniae

Streptococcus pyogenes

Swine vesicular disease

Toxoplasma gondii

Treponema pallidum

Trichomonas/
Tritrichomonas

Trypanosoma cruzi

Trypanosoma evansi

Vaccinia

Valley Fever

Vesicular stomatitis

Vibrio

West Nile virus

White nose syndrome

Yersinia enterocolitica

Yersinia pestis

Yersinia pseudotuberculosis


Pasteurella multocida PCR test

wildlife and zoo assay data sheet

Pasteurella multocida

Test code: B0045 - Ultrasensitive qualitative detection of Pasteurella multocida by real time PCR

 

Pasteurella bacteria are small, nonmotile, gram-negative, bipolar-staining bacilli that normally inhabit the nasal, gingival and tonsillar regions of most domestic cats, many dogs and many other animal species. P. multocida is also associated with hemorrhagic septicemia in elephants.

Pasteurella secretes an endotoxin that changes the properties of the pulmonary surfactant. This alters pulmonary mechanics and gas exchange, often resulting in a pneumonia which is slow to resolve. Abscesses or pleuritis may also result from Pasteurella infection.

Pasteurella multocida is the most common respiratory pathogen in the domestic rabbit. Infection with the bacteria can result in rhinitis, conjunctivitis, pneumonia, abscesses, genital tract infections, and septicemia. The bacteria can be transmitted via aerosol or contact with an infected animal, either directly or through fomites. P. multocida initially colonizes the pharynx, then moves to the nasal cavity and surrounding tissue, with the potential to spread to the rest of the body. Colonization of the nasal cavity may take two weeks to occur, at which time clinical signs may or may not appear. Infected rabbits may become carriers without exhibiting clinical signs.

Traditionally, diagnosis of Pasteurella was based on clinical findings, culture and/ or serological testing. Although culture identification methods are definitive, they are time consuming and costly. False-negative culture results are frequently observed due to the fact that P. multocida dies easily during transport to the laboratory or is overgrown by other bacteria (nasal flora and contaminants) in the culture.

Serology can be used for cases when infection is suspected in organs for which cultures are not attainable, or when culturing has yielded no results. However, a seropositive titer to P. multocida merely indicates past exposure to the organism. Because many rabbits and other animals have been exposed to this organism, a diagnosis of pasteurellosis cannot be made based on serologic results alone. Molecular detection by PCR, however, offers a highly sensitive, accurate and timely method for detecting Pasteurella and confirming current infection (Miflin and Blackall, 2001).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Pasteurella
  • Help ensure that rabbit facilities are free of Pasteurella
  • Early prevention of spread of Pasteurella among a facility
  • Minimize personnel exposure to Pasteurella
  • Safety monitoring of biological products that derive from rabbits and other animals

References:
Miflin, J. K. and Blackall, P. J. (2001) Development of a 23 S rRNA-based PCR assay for the identification of Pasteurella multocida. Lett. Appl. Microbiol. 33: 216–221.

Specimen requirements: Nasal swab or 0.2 ml whole blood in EDTA.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time polymerase chain reaction

Normal range: Nondetected

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