We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

Parrots moving in or moving out? Try our psittacine PCR screening panel.

Respiratory problems got you breathless? Try our poultry respiratory PCR panel.

Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere because DRY CARD samples are small, light and stable at room temperature for several weeks.

Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea

Brachyspira pilosicoli

Brucella

Cache Valley virus

Campylobacter      

Candida

Caprine arthritis-encephalitis (CAE) virus

Chlamydophila psittaci

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Cowpox

Coxiella burnetii

Cryptococcus

Cryptosporidium

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Foot and mouth disease

Fowlpox

Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Actinobacillus pleuropneumoniae PCR test for pigs
avian & livestock assay data sheet

Actinobacillus pleuropneumoniae

Test code:
B0099 - Ultrasensitive qualitative detection of Actinobacillus pleuropneumoniae by real time polymerase chain reaction.

Actinobacillus pleuropneumoniae, previously known as Haemophilus pleuropneumoniae, causes respiratory infection of swine. There are at least twelve different strains, some of which are non-pathogenic, but others can cause severe disease symptoms. Strains 1, 5, 9, 11 and 12 are highly virulent, while strains 3 and 6 cause mild disease symptoms. The organism shows affinity for the tonsils and respiratory tract of infected animals. The incubation period is usually very short, from as little as 12 hours to 3 days. Infected animals can spread the bacteria through respiratory aerosols, and the bacteria can survive in discharges, serum, and tissues for up to 5 days.

The major threat of this bacterial infection is that some pigs can carry the bacteria without any clinical signs. Young piglets with poorly developed immune system are particularly susceptible to infection. Once piglets are infected, rapid onset of pneumonia can occur and may result in hemorrhagic pneumonia. Prior to death or just after death, it is common to see blood coming from the nasal cavity of pigs. Postmortem examination can reveal characteristic lesions: large areas of hemorrhage in the dorsal lobes of the lungs. Lesions of this type and location do not commonly result from other infections - most other microbial infections cause lesions in the lower lung lobes, or throughout the lungs without hemorrhage.

The ability to detect these bacteria in subclinically infected herds is essential for control of the disease. Serology, and both standard and immunomagnetic separation-based (IMS) bacterial isolation methods, have sometimes been used for this purpose. Serological monitoring is cheap but it has limitations: infected pigs may be serologically negative, and sometimes, inconclusive serological results may be observed in the absence of clinical signs or pathological lesions. Culture identification of the organism or antigen detection by immunological methods usually has very low sensitivity. However, molecular detection by PCR is rapid, sensitive and specific, and is an important alternative to traditional methods in identifying these bacteria (Frey, J., 2003).

Utilities:

  • Help confirm the disease causing agent
  • Help ensure that swine herds are free of Actinobacillus pleuropneumoniae bacteria
  • Early prevention of spread of the bacteria among a herd
  • Minimize human exposure to the bacteria

References:
Frey, J. (2003) Detection, identification, and subtyping of Actinobacillus pleuropneumoniae. Methods Mol. Biol. 216:87-95.

Specimen requirements: Nasopharyngeal swab, or environmental swab, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml serum, or 0.2 ml fresh, frozen or fixed tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

2003-2016 Zoologix, Inc. • Email Zoologix • Phone (818) 717-8880