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Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Avian adenovirus

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Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

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Bovine endogenous retrovirus

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Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

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Bovine viral diarrhea

Brachyspira pilosicoli

Brucella

Cache Valley virus

Campylobacter      

Candida

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Chlamydophila psittaci

Classical swine fever

Clostridium

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Coxiella burnetii

Cryptococcus

Cryptosporidium

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Erysipelothrix rhusiopathiae

Foot and mouth disease

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Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

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Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma species

Mycoplasma suis

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Valley fever

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Histoplasma PCR test for birds and livestock

avian & livestock assay data sheet

Histoplasma

Test code:
F0006 - Ultrasensitive qualitative detection of Histoplasma species by real time polymerase chain reaction

Histoplasma is a genus of dimorphic fungi belonging to the family Ajellomycetaceae which are commonly found in bird and bat fecal material. The Histoplasma genus includes H. capsulatum which causes histoplasmosis; H. farciminosum which causes epizootic lymphangitis in horses; and H. duboisii which causes African histoplasmosis.

H. capsulatum is most prevalent in the Ohio and Mississippi river valleys. The disease caused by H. capsulatum is called histoplasmosis, also known as "cave disease," "Darling's disease," "Ohio Valley disease," "Reticuloendotheliosis," "spelunker’s lung" and “caver's disease.” Symptoms primarily affect the lungs, but other organs can be affected if the fungus spreads in the body. Histoplasmosis is commonly found in immunocompromised individuals such as AIDS patients and cancer patients.

People can be infected by inhaling microscopic fungi borne from bird or bat feces, or decomposing human biological fluids including urine, vomit, and feces.

Histoplasmosis can be diagnosed by detection of antigens in blood or urine samples by immunological or molecular methods. However, immunology is not very specific because antigens of Histoplasma can cross-react with antigens of blastomycosis, coccidioidomycosis, paracoccidioidomycosis and Penicillium marneffei infection. Formal histoplasmosis diagnoses may be confirmed by culturing the fungus directly. However, cultures may take up to 6 weeks for diagnostic growth to occur. Molecular detection by polymerase chain reaction (PCR) can provide a rapid, specific and sensitive method for diagnosis of this fungus (Elias et al., 2012).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Histoplasma infection.
  • Help ensure that flocks and animal groups are free of this fungus
  • Early prevention of spread of this fungus
  • Minimize personnel exposure to this fungus
  • Safety monitoring of biological products and vaccines that derive from birds and susceptible livestock

References:
Elías, N.A., Cuestas, M.L., Sandoval, M., Poblete, G., Lopez-Daneri, G., Jewtuchowicz, V., Iovannitti, C. and Mujica, M.T. (2012) Rapid identification of Histoplasma capsulatum directly from cultures by multiplex PCR. Mycopathologia. 174:451-456.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or urine, or sputum, or bronchoalveolar lavage, or feces or fecal swab, or 0.2 ml fresh, frozen or fixed tissue, or environmental surface swab.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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