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...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Infectious laryngotracheitis PCR test for poultry
avian & livestock assay data sheet

Infectious laryngotracheitis (ILT) virus

Test code:
S0085
- Ultrasensitive qualitative detection of infectious laryngotracheitis virus by real time polymerase chain reaction.

S0085 is included in the poultry respiratory panel

Avian infectious laryngotracheitis (ILT) is a respiratory disease caused by Herpesviridae alphaherpesvirinae gallid herpesvirus 1, also known as infectious laryngotracheitis virus. The virus mainly causes respiratory disease in chickens, but it can also infect pheasants, partridges and peafowl.

Clinically, ILT may appear in three forms:  peracute, subacute, and chronic or mild. In the peracute form, onset of disease is very sudden and may spread among a flock very quickly. Morbidity is high and mortality can exceed 50%. In the subacute form, the onset of illness is slower and infected chickens may develop respiratory symptoms for several days before dying. Morbidity is high but mortality is lower than in the peracute form, between 10% and 30%. The chronic or mild form of the disease has a much lower mortality rate, 1 to 2%, with most affected birds dying of suffocation. However, the birds with this chronic form of the disease may become carriers and re-excrete the virus without clinical signs.

Diagnosis of this infection has been done by virus isolation or serological neutralization techniques in the past. However, molecular detection by PCR has been shown to be more sensitive than other methods in identifying the virus (Alexander and Nagy, 1997; Williams, et al., 1994).

Utilities:

  • Help confirm the disease causing agent
  • Environmental monitoring
  • Help ensure that bird populations are free of ILTV
  • Early prevention of spread of this virus among bird populations
  • Minimize human exposure to this virus
  • Safety monitoring of biological products and vaccines that derive from birds

References:
Alexander,  H.S. and Nagy, E. (1997). Polymerase chain reaction to detect infectious laryngotracheitis virus in conjunctival swabs from experimentally infected chickens. Avian Dis., 41: 646–653.

Williams, R.A., Savage, C.E. and Jones, R.C. (1994). A comparison of direct electron microscopy, virus isolation and a DNA amplification method for the detection of avian infectious laryngotracheitis virus in field material. Avian Pathol., 23: 709–720.

Specimen requirements: Tracheal swab, or 0.2 ml fresh, frozen or preserved tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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