We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

Parrots moving in or moving out? Try our psittacine PCR screening panel.

Respiratory problems got you breathless? Try our poultry respiratory PCR panel.

Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere because DRY CARD samples are small, light and stable at room temperature for several weeks.

Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine endogenous retrovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea

Brachyspira pilosicoli

Brucella

Cache Valley virus

Campylobacter      

Candida

Caprine arthritis-encephalitis (CAE) virus

Chlamydophila psittaci

Classical swine fever

Clostridium

Coccidia

Coccidiodes

Coronaviruses

Cowpox

Coxiella burnetii

Cryptococcus

Cryptosporidium

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Erysipelothrix rhusiopathiae

Foot and mouth disease

Fowlpox

Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma species

Mycoplasma suis

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Valley fever

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Mycoplasma suis PCR test
avian & livestock assay data sheet

Mycoplasma suis

Test code:  B0109 - Ultrasensitive qualitative detection of Mycoplasma suis by real time polymerase chain reaction.

Mycoplasma suis, previously known as Eperythrozoonosis suis or Mycoplasma haemosuis, is an obligate intracellular bacteria. The organism is approximately 0.8 to 1.0 um in diameter but may be larger during the acute stage of infection. It is predominantly coccoid-shaped but may appear rod or ring-shaped when viewed on an erythrocyte’s cell membrane.

Infection with M. suis can occur in all age groups of swine but symptoms occur mostly in young growing pigs. Disease symptoms include listlessness, fever, anorexia, hemolytic anemia and, in severe cases, jaundice. Serologic survey shows that exposure to this bacterial infection is less than 15% among swine populations, and only a small number of outbreaks have been reported. The outbreaks usually occur when animals are stressed.

However, many adult pigs could be carriers, as infection in this age group is usually subclinical. Since the disease occurs more frequently in the summer, mosquitoes and biting flies, as well as the hog louse (Haematopinus suis), are suspected to be vectors of transmission. The use of contaminated surgical instruments including castration knives, and needles used repeatedly during vaccination, can also transmit the bacteria.

Direct examination of blood smears has been used to diagnose this disease, but this method has a low sensitivity and is not very specific. Serologic testing is slow, and cross-reactivity with other Mycoplasma species can cause false positive serology results. Molecular detection by polymerase chain reaction is highly specific and sensitive, and is rapid. PCR detection of this bacterium is now considered the method of choice (Ritzmann et al., 2009).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm clinical diagnosis of M. suis infection
  • Help ensure that herds are free of M. suis
  • Early prevention of spread of M. suis among and between herds
  • Minimize human exposure to these bacteria
  • Safety monitoring of biological products that derive from pigs

References:
Ritzmann, M., Grimm, J., Heinritzi, K., Hoelzle, K. and Hoelzle, L.E. (2009) Prevalence of Mycoplasma suis in slaughter pigs, with correlation of PCR results to hematological findings. Vet. Microbiol. 133:84–91.

Preferred specimen: 0.2 ml feces; or fecal swab; or oral swab; or 0.2 ml whole blood in EDTA (purple top) tube; or 0.2 ml fresh, frozen or fixed tissue; or 0.2 ml cell culture; or environmental swab.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

2003-2017 Zoologix, Inc. • Email Zoologix • Phone (818) 717-8880