& livestock assay data sheet
Bovine herpesvirus type 2 (BoHV2)
- Ultrasensitive detection of bovine herpesvirus 2 by real time PCR
Bovine herpesvirus type 2 (BoHV-2) is also known as bovine mammillitis
virus. It belongs to subfamily
Infection of cows with this virus may result in an acute, ulcerative
condition of teat and udder skin referred to as bovine
ulcerative mammillitis. This often results in reduced milk
production and increased susceptibility to bacterial mastitis.
However, not all infected cows show acute symptoms; infected
animals may develop clinical symptoms ranging from relatively
mild, small plaques of edema to severe ulceration. Early signs
vary among infected animals, but the lesions often begin as one
or more thickened, edematous plaques of varying size on the skin
of one or more teats. Vesicles develop and may rapidly rupture,
leaving a raw, ulcerated area that becomes covered with a
dark-colored scab. The scabs tend to crack and bleed, especially
if milking is attempted. Because the infection causes teats to
be painful, infected cows often resist milking, leading to
development of mastitis.
The greatest incidence is often seen in first-lactation cows, but
previously unexposed cows of any age are susceptible. Severe
lesions may take several weeks to heal. Infected animals may
become latent carriers, and re-activation of the virus may
Viral isolation has been used to diagnose this infection, but culture
detection is not definitive. Serological detection requires time
for infected animals to develop a detectable immune response.
However, molecular detection by PCR is rapid, sensitive and
specific, and is a good alternative to traditional methods
(Campos et al., 2014).
Help confirm the disease causing agent
Identify BoHV2 virus carriers
Help ensure that herds are free of BoHV2
Early prevention of spread of the virus among animals
Minimize human exposure to the virus
Safety monitoring of biological products that derive
Campos, F.S., Franco, A.C., Oliveira, M.T., Firpo, R., Strelczuk, G.,
Fontoura, F.E., Kulmann, M.I., Maidana, S., Romera, S.A.,
Spilki, F.R., Silva, A.D., Hübner, S.O. and Roehe, P.M. (2014)
Detection of bovine herpesvirus 2 and bovine herpesvirus
4 DNA in trigeminal ganglia of naturally infected cattle by
polymerase chain reaction. Vet. Microbiol. 171:182-188.
whole blood in EDTA (purple top) or ACD (yellow top) tube, or
lesion swab or scab, or 0.2 ml milk, or 0.2 ml fresh, frozen or fixed
Contact Zoologix if advice is needed to determine an appropriate specimen type for a specific diagnostic application. For specimen types not listed here, please contact Zoologix to confirm specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See
for more information.
2 business days
Qualitative real time PCR