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Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

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Avian adenovirus

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Blood typing for swine

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Brachyspira pilosicoli

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Campylobacter      

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Chlamydophila psittaci

Classical swine fever

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Edwardsiella

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Ornithobacterium rhinotracheale

Ovine herpesvirus 2

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Peste des petits ruminants virus (PPRV)

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Plasmodium species

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Porcine circovirus 1

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Poultry respiratory panel

Pseudocowpox

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Q fever

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Staphylococcus xylosus

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Streptococcus

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Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Avian and mammalian Staphylococcus xylosus PCR test

avian & livestock assay data sheet

Staphylococcus xylosus

Test code:
B0094 - Ultrasensitive qualitative detection of Staphylococcus xylosus bacteria by real time polymerase chain reaction

Members of the Staphylococcus genus are gram-positive bacteria that form clusters of cells. Like most staphylococcal species, Staphylococcus xylosus is coagulase-negative and non-hemolytic, and is usually found as normal flora on the skin of humans and animals, and in the environment.

Although S. xylosus generally does not cause disease in animals or humans, it has infrequently been implicated in some disease cases, such as nasal dermatitis in gerbils, pyelonephritis and endocarditis in humans, avian staphylococcosis, and bovine intermammary infections.

S. xylosus is a source of proteolytic enzymes commonly used in food processing. It is essential in making sausage and some cheeses. Its growth is not affected by yeasts during the processing of cheese, and its proteolytic enzymes and lecithinases improve the flavor and tenderness of sausage.

Traditionally, culture identification of S. xylosus required laborious colony isolation and biochemical analysis because Staphylococcus bacteria are ubiquitously present in the environment. Molecular detection by polymerase chain reaction (PCR) is rapid, sensitive and specific, and is a useful alternative to the traditional method (Blaiotta et al., 2003).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Staphylococcus xylosus infection.
  • Help ensure that flocks are free of Staphylococcus xylosus
  • Early prevention of spread of Staphylococcus xylosus
  • Minimize personnel exposure to these bacteria
  • Safety monitoring of biological products and vaccines that derive from susceptible birds or mammals

Reference:
Blaiotta, G., Pennacchia, C., Parente, E. and Villani, F. (2003) Design and evaluation of specific PCR primers for rapid and reliable identification of Staphylococcus xylosus strains isolated from dry fermented sausages. Syst. Appl. Microbiol. 26:601-610.

Specimen requirements:  0.2 ml whole blood in eDTA (purple top) or ACD (yellow top) tube, or nsapharyngeal swab, or 0.2 ml culture, or environental swab, or food swab.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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