We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

Parrots moving in or moving out? Try our psittacine PCR screening panel.

Respiratory problems got you breathless? Try our poultry respiratory PCR panel.

Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere because DRY CARD samples are small, light and stable at room temperature for several weeks.

Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine endogenous retrovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea

Brachyspira pilosicoli

Brucella

Cache Valley virus

Campylobacter      

Candida

Caprine arthritis-encephalitis (CAE) virus

Chlamydophila psittaci

Classical swine fever

Clostridium

Coccidia

Coccidiodes

Coronaviruses

Cowpox

Coxiella burnetii

Cryptococcus

Cryptosporidium

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Erysipelothrix rhusiopathiae

Foot and mouth disease

Fowlpox

Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma species

Mycoplasma suis

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Valley fever

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Porcine respiratory coronavirus PCR test
avian & livestock assay data sheet

Porcine respiratory coronavirus (PRCV)

Test code: S0214 - Ultrasensitive detection of porcine respiratory coronavirus by reverse transcription coupled real time PCR

Porcine respiratory coronavirus (PRCV) is a deletion mutant of transmissible gastroenteritis virus (TGEV). The deletion occurs in the spike (S) gene of TGEV. PRCV is classified as a group 1 coronavirus.

Infection of neonatal piglets with this virus can result in acute epidemic diarrhea. Although most PRCV infections of pigs are mild or subclinical, concurrent or secondary infections may cause severe symptoms. PRCV infection of young pigs is associated with transient viremia, often with extremely high titers of PRCV in the lungs. However, the various strains of PRCV have different affinities for respiratory system tissue. In many cases, the damage does not result in clinical disease and the most virulent strains generally only result in mild clinical disease.

Diagnosis of PRCV-infection is not easy because many viruses can cause similar respiratory symptoms in swine, such as swine influenza virus (SIV), porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), porcine cytomegalovirus (PCMV), porcine paramyxovirus (PPMV), hemagglutinating encephalomyelitis virus (HEV), encephalomyocarditis virus (EMC), porcine parvovirus (PPV), porcine adenovirus, and porcine enterovirus.

Culture detection of PRCV is not suitable because the virus does not grow well in culture. Serological detection is also not suitable because a significant percentage of pigs may have prior exposure to the virus and confirmation of infection requires measurement of paired titers several weeks apart. Molecular detection by polymerase chain reaction is rapid, sensitive and specific, and is a good alternative to the traditional methods (Usami et al., 2008).

Utilities:

  • Help confirm the disease causing agent
  • Identify PRCV carriers
  • Help ensure that herds are free of PRCV
  • Early prevention of spread of the virus among animals
  • Minimize human exposure to the virus
  • Safety monitoring of biological products that derive from animals

References:
Usami, Y., Fukai, K., Ichikawa, Y., Okuda, Y., Shibata, I., Motoyama, C., Imai, K. and Kirisawa, R. (2008) Virological and serological studies of porcine respiratory coronavirus infection on a Japanese farm. J. Vet. Med. Sci. 70:929-936.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or nasal swab, or 0.2 ml feces, or rectal swab, or 0.2 ml fresh or frozen tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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