We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

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Respiratory problems got you breathless? Try our poultry respiratory PCR panel.

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Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea

Brachyspira pilosicoli

Brucella

Cache Valley virus

Campylobacter      

Candida

Caprine arthritis-encephalitis (CAE) virus

Chlamydophila psittaci

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Cowpox

Coxiella burnetii

Cryptococcus

Cryptosporidium

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Foot and mouth disease

Fowlpox

Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Porcine cytomegalovirus PCR test
avian & livestock assay data sheet

Porcine cytomegalovirus

Test code:
S0128 - Ultrasensitive detection of porcine cytomegalovirus by real time PCR

 

Herpesviruses are widely distributed and have been found in insects, reptiles, amphibians and every species of bird and mammal. One important characteristic of herpesvirus infection is that the virus persists in the infected host for life and is frequently reactivated and shed. In pigs, five herpesviruses have been identified: three recently identified lymphotrophic herpesviruses, pseudorabies virus and porcine cytomegalovirus (PCMV).

PCMV causes inclusion body rhinitis and abortion or neonatal piglet losses in pigs. On microscopic examination, CMV infection causes large intranuclear inclusion bodies in infected cells. In pigs, a major site of infection tends to be the turbinates and the rest of the upper respiratory tract. Clinically, inclusion body rhinitis is often confused with atrophic rhinitis, another upper respiratory tract disease of multiple etiologies.

Like human CMV, porcine CMV crosses the placenta and infects fetuses, with resulting congenital infections. In susceptible herds, infection with PCMV can lead to fetal and piglet death, runting, rhinitis, pneumonia, and poor weight gain. In herds where management conditions tend to be good or exceptional, the virus may be endemic without causing any apparent clinical disease or economic loss. However, these infected animals can be latent carriers of the virus.

Antibodies to this virus have been found in a high percentage of swine herds worldwide. Because of the high prevalence of positive serology, serological identification of infected pigs is not useful. Many latent carriers remain unidentified, posing serious problems with research using the pig as a model. In xenotransplantation between pig and human, reactivation of the latent virus can cause postransplantation failure. Molecular detection of the virus is an important tool that can provide rapid, sensitive and specific detection of the viral nucleic acid in suspected animals (Hamel et al., 1999).

Utilities:

  • Help confirm the disease causing agent
  • Identify PCMV carriers
  • Help ensure that animal colonies and populations are free of PCMV
  • Early prevention of spread of the virus among animals
  • Minimize human exposure to the virus
  • Safety monitoring of biological products that derive from animals

References:
Hamel, A.L., Lin, L., Sachvie, C., Grudeski, E., and Nayar, G.P.S. (1999) Assay for Detecting Porcine Cytomegalovirus. J Clin Microbiol. 37: 3767–3768.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml fresh, frozen or fixed tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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