We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

Parrots moving in or moving out? Try our psittacine PCR screening panel.

Respiratory problems got you breathless? Try our poultry respiratory PCR panel.

Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere because DRY CARD samples are small, light and stable at room temperature for several weeks.

Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea

Brachyspira pilosicoli

Brucella

Cache Valley virus

Campylobacter      

Candida

Caprine arthritis-encephalitis (CAE) virus

Chlamydophila psittaci

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Cowpox

Coxiella burnetii

Cryptococcus

Cryptosporidium

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Erysipelothrix rhusiopathiae

Foot and mouth disease

Fowlpox

Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Rinderpest PCR test
avian & livestock assay data sheet

Rinderpest

Test code: S0205 - Ultrasensitive detection of Rinderpest virus by reverse transcription coupled real time PCR

Rinderpest virus (RPV) is an RNA virus belonging to the genus Morbillivirus of the family Paramyxoviridae. Although there is only one serotype of RPV, different strains of this virus vary greatly in their pathogenicity, from those causing 100% mortality, as seen with the Saudi/81 strain, to others in which infection causes no detectable clinical signs.

Rinderpest disease, also known as cattle plague, is a contagious viral disease affecting clovenhoofed animals, mainly cattle and buffalo. Many species of wild and domestic cloven-hoofed animals, including sheep and goats, only develop mild symptoms when infected, but the mortality rate can reach up to 100 per cent in highly susceptible cattle or buffalo herds. Infected animals can develop fever, necrotic stomatitis, gastroenteritis and lymphoid necrosis.

Transmission of the virus requires close contact between animals. The virus is present in small amounts of nasal and ocular secretions 1–2 days before fever begins; levels becomes high in secretions and excretions during the first week of clinical disease and decrease rapidly as animals develop specific antibodies and begin to recover.

Recovered pigs may remain chronically infected and excrete the virus for 6 weeks after infection. Contaminated pens and garbage feeding, particularly with material from international airports or seaports, are documented methods of spread due to the resistance of the virus to inactivation.

Laboratory tests are important to differentiate the disease from bovine viral diarrhea in particular, as well as East Coast fever, foot-and-mouth disease, infectious bovine rhinotracheitis, and malignant catarrhal fever. Viral isolation and detection of specific viral antigens in affected tissues using an immunodiffusion test was formerly the standard, but simpler, more rapid and more discriminating tests, such as immune capture ELISA and reverse transcription coupled real time PCR, are increasingly being used as the first line of diagnosis. (Carrillo et al., 2010).

Utilities:

  • Help confirm the disease causing agent
  • Identify Rinderpest virus carriers
  • Help ensure that animal herds and populations are free of Rinderpest
  • Early prevention of spread of the virus among animals
  • Minimize human exposure to the virus
  • Safety monitoring of biological products that derive from animals

References:
Carrillo, C., Prarat, M., Vagnozzi, A., Calahan, J.D., Smoliga, G., Nelson, W.M. and Rodriguez, L.L. (2010) Specific detection of Rinderpest virus by real-time reverse transcription-PCR in preclinical and clinical samples from experimentally infected cattle. J. Clin. Microbiol. 48:4094-4101.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or nasal swab, or 0.2 ml feces, or rectal swab, or 0.2 ml fresh or frozen tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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