& livestock assay data sheet
Teschovirus (Teschen-Talfan disease)
detection of porcine teschovirus serotype 1 by reverse transcription
coupled real time PCR
Porcine teschovirus (PTV) is a picornavirus of swine that can cause a
wide range of clinical signs. This virus only infect pigs. There
are at least 11 distinct serotypes of PTV, PTV-1 through PTV-11.
Most of the 11 serotypes are widely distributed and infect pigs
either asymptomatically or cause disease only in young animals
which do not have well-developed immune responses. However, some
strains of the most virulent serotype (PTV-1) can cause
teschovirus encephalomyelitis, a highly contagious and fatal
neurological disease of pigs. This fatal disease was formerly
called Teschen disease, but is now called teschovirus
Other strains of PTV-1, as well as other PTV serotypes, can cause milder
disease or inapparent infections in pigs. These milder diseases
have a variety of names including Talfan disease, benign
enzootic paresis and poliomyelitis suum.
Upon ingestion, porcine teschovirus multiplies in the gastrointestinal
tract and associated lymphoid tissues, including the tonsils.
Virus can then be shed in feces and oral secretions.
Convalescent animals can excrete virus in feces for up to 7
weeks. Porcine teschoviruses are readily spread on fomites, and
may be transmitted in inadequately heat-treated pig swill. The
virus can survive in the environment for more than 5 months at
15ºC (59ºF) and can also survive for prolonged periods in liquid
Help confirm the disease causing agent
Identify PTV-1 carriers
Help ensure that herds and populations are free of this
Early prevention of spread of the virus among animals
Minimize human exposure to the virus
Safety monitoring of biological products that derive
La Rosa, G., Muscillo, M., Di Grazia, A., Fontana, S., Iaconelli, M. and
Tollis, M. (2006) Validation of rt-PCR assays for molecular
characterization of porcine teschoviruses and enteroviruses. J.
Vet. Med. B. Infect. Dis. Vet. Public Health 53:257-265.
whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml feces, or rectal swab, or 0.2
ml CSF, or 0.2 ml urine, or 0.2 ml fresh or frozen tissue.
types other than those listed here, please call to confirm
specimen acceptability and shipping instructions.
specimen types, if there will be a delay in shipping, or during
very warm weather, refrigerate specimens until shipped and ship
with a cold pack unless more stringent shipping requirements are
specified. Frozen specimens should be shipped so as to remain
frozen in transit. See
for more information.
2 business days
Qualitative real time PCR