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Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea

Brachyspira pilosicoli

Brucella

Cache Valley virus

Campylobacter      

Candida

Caprine arthritis-encephalitis (CAE) virus

Chlamydophila psittaci

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Cowpox

Coxiella burnetii

Cryptococcus

Cryptosporidium

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Foot and mouth disease

Fowlpox

Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

avian & livestock assay data sheet

Porcine parvovirus

Test code:
S0122 - Ultrasensitive qualitative detection of porcine parvovirus by real time polymerase chain reaction

 

Porcine parvovirus (PPV) is a member of the Parvoviridae family. Swine infected with the virus can experience reproductive problems, including abortion, small litters, still births, neonatal deaths and weak piglets. There is no clinically apparent disease in non-pregnant pigs. Disease usually occurs when sero-negative dams are infected in the first half of gestation and the virus crosses the placenta.

Infection of post-natal pigs usually produces no detectable clinical signs. Even in pigs showing symptoms, the clinical picture is variable depending on the developmental stage. There may be increased abortion rates in herds, small litters, mummified fetuses, neonatal death and weak piglets. In boars, PPV can temporarily disturb spermatogenesis.

PPV is endemic in most countries with large pig populations. The virus can be transmitted via oronasal, transplacental and venereal routes, but oronasal is the most important route of infection. The virus is shed for only about two weeks after infection, in feces, urine, semen and nasal secretions. The greatest source of infection is the fluids and fetal membranes of parturient sows. The virus can persist for four months or more in the environment.

The most common methods used to detect PPV infection are fluorescent antibody (IFA) staining of fetal tissues, hemagglutination assay of tissue extracts and virus isolation from fetal tissues. Low specificity and low sensitivity are the major drawbacks of these techniques. In contrast, molecular detection by PCR offers rapid, specific and sensitive detection of the virus (Soares et al., 1999).

Utilities:

  • Help confirm the disease causing agent
  • Identify porcine parvovirus carriers
  • Help ensure that animal groups and populations are free of porcine parvovirus
  • Early prevention of spread of the virus among animals
  • Minimize human exposure to the virus
  • Safety monitoring of biological products that derive from animals

References:
Soares, R.M., Durigon, E.L., Bersano, J.G. and Richtzenhain LJ. (1999) Detection of porcine parvovirus DNA by the polymerase chain reaction assay using primers to the highly conserved nonstructural protein gene, NS-1. J. Virol. Methods 78: 191-198.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml feces or urine, or 0.2 ml fresh, frozen or fixed tissue, or nasal swab or fecal swab.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

Porcine parvovirus PCR test

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