We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

Parrots moving in or moving out? Try our psittacine PCR screening panel.

Respiratory problems got you breathless? Try our poultry respiratory PCR panel.

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Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine endogenous retrovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea

Brachyspira pilosicoli

Brucella

Cache Valley virus

Campylobacter      

Candida

Caprine arthritis-encephalitis (CAE) virus

Chlamydophila psittaci

Classical swine fever

Clostridium

Coccidia

Coccidiodes

Coronaviruses

Cowpox

Coxiella burnetii

Cryptococcus

Cryptosporidium

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Erysipelothrix rhusiopathiae

Foot and mouth disease

Fowlpox

Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma species

Mycoplasma suis

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Valley fever

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Infectious bursal disease PCR test for poultry
avian & livestock assay data sheet

 

Infectious bursal disease virus (IBDV)

Test code:
S0084
- Ultrasensitive qualitative detection of infectious bursal disease virus by reverse transcription coupled real time polymerase chain reaction.

Infectious bursal disease virus (IBDV), which causes the notorious infectious bursal disease, is a birnavirus that can be readily isolated from the bursa of Fabricius of infected birds, and sometimes also from other organs. Infected birds can shed the virus in their feces. The virus is very stable in the environment and can readily be transmitted on fomites, so it is difficult to eradicate from premises.

IBD is very contagious. Symptoms depend on age, breed, and virulence of the strain of the virus. Infections may be subclinical or clinical. Chickens are most susceptible to the infection at 3-6 weeks of age, but severe infections have occurred in Leghorn chickens up to 18 weeks old. Infections before 3 weeks of age are usually subclinical.

Early subclinical infections are the most economically important form of the disease. They cause severe, long-lasting immunosuppression due to destruction of immature lymphocytes in the bursa of Fabricius, thymus, and spleen. Growth is significantly reduced, and even subclinical infected chickens can spread the disease.

In clinical infections, onset of the disease is sudden, usually 3-4 days after contact with the virus. Chickens exhibit severe prostration, incoordination, watery diarrhea, soiled vent feathers, vent picking, and inflammation of the cloaca. Mortality rate can exceed 20%.  Infected chickens may recover in less than 1 week after the onset of the symptoms, with broiler weight gain delayed by 3-5 days. The presence of maternal antibody will modify the clinical course of the disease.

Traditional methods for diagnosis of IBDV infection include agar gel precipitation, virus neutralization, and enzyme-linked immunosorbent assay (ELISA). These tests are designed to measure levels of antibodies to IBDV. However, these techniques are not very sensitive, and the presence of maternal antibody may complicate interpretation. Molecular detection by PCR can provide rapid, sensitive and specific detection of the virus (Lee et al., 1994).

Utilities:

  • Help confirm the disease causing agent
  • Environmental monitoring
  • Help ensure that flocks are free of IBDV
  • Early prevention of spread of the virus among flocks
  • Minimize human exposure to the virus
  • Safety monitoring of biological products and vaccines that derive from poultry

References:
Lee, L. H.,  Ting,  L. J., Shien, J. H and Shieh, H. K. (1994) Single-tube, noninterrupted reverse transcription-PCR for detection of infectious bursal disease virus. J. Clin. Microbiol. 32: 1268-1272.

Specimen requirements: 0.2 ml feces, or cloacal swab, or respiratory swab, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml fresh, frozen or fixed tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Reverse transcription coupled real time PCR

Normal range: Nondetected

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