We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

Parrots moving in or moving out? Try our psittacine PCR screening panel.

Respiratory problems got you breathless? Try our poultry respiratory PCR panel.

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Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine endogenous retrovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea

Brachyspira pilosicoli

Brucella

Cache Valley virus

Campylobacter      

Candida

Caprine arthritis-encephalitis (CAE) virus

Chlamydophila psittaci

Classical swine fever

Clostridium

Coccidia

Coccidiodes

Coronaviruses

Cowpox

Coxiella burnetii

Cryptococcus

Cryptosporidium

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Erysipelothrix rhusiopathiae

Foot and mouth disease

Fowlpox

Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma species

Mycoplasma suis

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Valley fever

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Pigeon circovirus PCR test
avian & livestock assay data sheet

Pigeon circovirus (PiCV)

Test code:
S0095 - Qualitative detection of pigeon circovirus by polymerase chain reaction

 

Pigeon circovirus (PiCV) infections have been described in several regions of the world. Infected pigeons may develop symptoms such as lethargy, anorexia, weight loss, reduced race performance, respiratory distress and diarrhea.

The viral genome of PiCV (also known as columbid circovirus or CoCV) has been found to share low-level DNA homology with psittacine beak and feather disease virus (BFDV). The virus currently is classified as a tentative member of family Circoviridae and the genome of PiCV is similar to other members of the family: It is a circular, single-stranded DNA of approximately 1.76–2.04 kb in size, contained within a non-enveloped protein coat.

Successful culture identification of virus has not been described. Histopathological examination of infected young birds reveals the presence of characteristic inclusions in lymphoid tissue such as the bursa of Fabricius. As this organ shrinks in size as birds grow older and is tiny or absent in adult birds, diagnosis by histopathology alone is therefore unreliable. Furthermore, this method does not allow early diagnosis of infection.

Molecular detection by PCR is very useful because it can identify the presence or absence of the viral genome in blood (Hattermann et al, 2002) and a number of different tissues (Todd et al, 2002), and can assist the post mortem diagnosis of PiCV disease. PCR detection of PiCV is highly sensitive and specific.

Utilities:

  • Help confirm the disease causing agent
  • Environmental monitoring
  • Help ensure that bird populations are free of PiCV
  • Early prevention of spread of the virus among bird populations
  • Minimize human exposure to the virus

References:
Hattermann, K., Soike, D., Grund, C. and Mankertz A, (2002) A method to diagnose Pigeon circovirus infection in vivo. J. Virol. Methods. 104:55-58.
Todd, D., Duchatel, J.P., Weston, J.H., Ball, N.W., Borghmans, B.J., Moffett, D.A. and Smyth, J.A. (2002) Evaluation of polymerase chain reaction and dot blot hybridization tests in the diagnosis of pigeon circovirus infections. Vet. Microbiol. 89:1-16.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml fresh, frozen or fixed tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative PCR

Normal range: Nondetected

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