We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

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Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea

Brachyspira pilosicoli

Brucella

Cache Valley virus

Campylobacter      

Candida

Caprine arthritis-encephalitis (CAE) virus

Chlamydophila psittaci

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Cowpox

Coxiella burnetii

Cryptococcus

Cryptosporidium

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Foot and mouth disease

Fowlpox

Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Swinepox PCR test
avian & livestock assay data sheet

Swinepox

Test code: S0212 - Ultrasensitive detection of swinepox virus (SWPV) by real time PCR

Swinepox virus (SWPV) is the only member of the Suipoxvirus genus of the poxviridae family of viruses, and is the etiologic agent of a worldwide disease specific to swine.

Infected pigs develop a skin disease characterized by generalized pustular lesions.  High rates of illness (occasionally >80%) can occur, although the mortality rate is low. The disease occurs mainly on farms with poor management and housing conditions, and primarily affects pigs less than 3 months of age; adult pigs show milder signs. The disease is transmitted by pig lice or through direct animal contact. Swinepox must be differentiated from a similar pustular disease of pigs caused by vaccinia virus; the two are difficult to distinguish clinically.

Swinepox begins with an incubation period of approximately one week. Small red areas then develop, most frequently on the face, ears, insides of the legs, and abdomen. These red areas than develop into papules and, within a few days, pustules or small vesicles may be seen. The centers of the pustules become dry and scabbed and are surrounded by a raised, inflamed zone so that the lesions appear umbilicated. Later, dark scabs of 1-2 cm diameter form. Affected piglets develop a “spotty” look. The scabs eventually drop or are rubbed off without leaving a scar. Successive bouts of lesions can occur, so that all lesions are not at the same stage. During early stages of the disease affected pigs may have mild fever, inappetence, and dullness.

Culture detection has been used in the past, but lacks sensitivity and cannot easily differentiate swinepox virus from vaccinia virus. However, molecular detection by PCR (Medaglia et al., 2011), is rapid, sensitive and specific, and is a good alternative to traditional methods.

Utilities:

  • Help confirm the disease causing agent
  • Help identify swinepox carriers
  • Help differentiate SWPV infections from vaccinia infections
  • Help ensure that herds and facilities are free of SWPV
  • Early prevention of spread of the virus among animals
  • Minimize human exposure to the virus
  • Safety monitoring of biological products that derive from animals

References:
Medaglia, M.L.G., Pereira, A. D. C., Freitas, T.R.P. and Damaso, C.R. (2011) Swinepox Virus Outbreak, Brazil, 2011. Emerg. Infect. Dis. 2011 17: 1976–1978.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or lesion swab, or scab, or 0.2 ml fresh, frozen or fixed tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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