We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

Parrots moving in or moving out? Try our psittacine PCR screening panel.

Respiratory problems got you breathless? Try our poultry respiratory PCR panel.

Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere because DRY CARD samples are small, light and stable at room temperature for several weeks.

Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea

Brachyspira pilosicoli

Brucella

Cache Valley virus

Campylobacter      

Candida

Caprine arthritis-encephalitis (CAE) virus

Chlamydophila psittaci

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Cowpox

Coxiella burnetii

Cryptococcus

Cryptosporidium

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Erysipelothrix rhusiopathiae

Foot and mouth disease

Fowlpox

Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Vaccinia virus PCR test
avian & livestock assay data sheet

Vaccinia virus

Test code: S0153
- Ultrasensitive detection of vaccinia virus by real time PCR

Vaccinia virus is a large, complex, enveloped virus belonging to the Poxvirus family. The virus contains a large, linear, double-stranded DNA genome with size close to 190 kb in length, which may encode for more than 250 genes. Members of the Poxvirus family are unique because they replicate only in the cytoplasmic region of the host cell and not in the nuclear region. Thus, the viral genome must be large enough to code for various proteins needed for viral DNA replication and gene transcription.

People infected with vaccinia virus usually develop very mild or subclinical symptoms. A few infected people may develop mild rash and fever. Immune responses generated from vaccinia infection can protect the infected person against subsequent smallpox infection due to the high degree of similarity between the two viruses. Vaccinia virus is often used as a live-virus vaccine against smallpox. However, certain complications and/or vaccine adverse effects occasionally arise from the use of this vaccine, especially in people whose immune systems are compromised.

Detection of vaccinia virus can be achieved by the shell vial culture method, but that method requires a lengthy incubation period and is not very sensitive. However, molecular detection by PCR is highly sensitive, specific and rapid (Fedorko et al., 2005).

Utilities:                         

  • Help confirm the disease causing agent
  • Identify vaccinia virus carriers
  • Screen research materials for the presence of vaccinia virus
  • Help ensure that animal groups are free of vaccinia virus
  • Early prevention of spread of the virus among animals
  • Minimize human exposure to the virus
  • Safety monitoring of biological products that derive from susceptible animals

References:
Fedorko, D.P., Preuss, J.C., Fahle, G.A., Li L, Fischer, S.H., Hohman, P. and Cohen, J.I. (2005) Comparison of methods for detection of vaccinia virus in patient specimens. J. Clin. Microbiol. 43:4602–4606.

Specimen requirements: 0.2 ml cell culture, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or lesion swab, or 0.2 ml fresh, frozen or preserved tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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