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Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine endogenous retrovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea

Brachyspira pilosicoli

Brucella

Cache Valley virus

Campylobacter      

Candida

Caprine arthritis-encephalitis (CAE) virus

Chlamydophila psittaci

Classical swine fever

Clostridium

Coccidia

Coccidiodes

Coronaviruses

Cowpox

Coxiella burnetii

Cryptococcus

Cryptosporidium

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Erysipelothrix rhusiopathiae

Foot and mouth disease

Fowlpox

Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma species

Mycoplasma suis

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Valley fever

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

avian & livestock assay data sheet

Porcine lymphotropic herpesviruses 1 and 2

Test code:
S0129 - Ultrasensitive detection of porcine lymphotropic herpesviruses 1 and 2 by real time PCR

 

Herpesviruses are widely distributed and have been found in insects, reptiles, amphibians and every species of bird and mammal. One important characteristic of herpesvirus infection is that the virus persists in the infected host for life and is frequently reactivated and shed. In pigs, five herpesviruses have been identified: pseudorabies virus, porcine cytomegalovirus (PCMV) and three recently identified lymphotrophic herpesviruses, PLHV-1, PLHV-2 and PLHV-3.

PLHV-1 and -2 are highly homologous to each other but not to PLHV-3. The two viruses are widespread in domestic pigs and are closely related to several ruminant gammaherpesviruses, most of which are etiologically implicated in the occurrence of malignant catarrhal fever (MCF), a lymphoproliferative inflammatory disease with an often fatal outcome. PLHV-1 and -2 are also related to Epstein-Barr Virus, Human Herpesvirus-8 and other gammaherpesviruses.

A recent study (McMahon et al., 2006) in domestic pigs has shown that PLHV1 infections are most common, being found in 74% of animals tested, followed by PLHV3 at 45% and PLHV2 at 21%. Infections with multiple PLHV species were frequently detected.

Like porcine CMV, antibodies to PLHV have been found in a high percentage of swine herds worldwide. Because of the high prevalence of positive serology, serological identification of infected pigs is not possible. Many of these latent carriers remain unidentified, posing serious problems for research using the pig as a model. In xenotransplantation between pig and human, reactivation of these latent viruses can cause postransplantation failure. Molecular detection of these viruses is an important tool to provide rapid, sensitive and specific detection of the viral nucleic acid in suspected animals.

Utilities:

  • Help confirm the disease causing agent
  • Identify PLHV-1 and -2 carriers
  • Help ensure that animal colonies and populations are free of PLHV-1 and -2
  • Early prevention of spread of these viruses among animals
  • Minimize human exposure to these viruses
  • Safety monitoring of biological products that derive from animals

References:
McMahon, K.J., Minihan, D., Campion, E.M., Loughran, S.T., Allan, G., McNeilly, F. and Walls, D. (2006) Infection of pigs in Ireland with lymphotropic herpesviruses and relationship to postweaning multisystemic wasting syndrome. Vet. Microbiol. 116:60-68.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml fresh, frozen or fixed tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

Porcine lymphotropic herpesvirus PCR test

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