We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

Parrots moving in or moving out? Try our psittacine PCR screening panel.

Respiratory problems got you breathless? Try our poultry respiratory PCR panel.

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Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea

Brachyspira pilosicoli

Brucella

Cache Valley virus

Campylobacter      

Candida

Caprine arthritis-encephalitis (CAE) virus

Chlamydophila psittaci

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Cowpox

Coxiella burnetii

Cryptococcus

Cryptosporidium

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Foot and mouth disease

Fowlpox

Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

avian & livestock assay data sheet

Porcine transmissible gastroenteritis virus (TGEV)

Test code:
S0167
- Ultrasensitive qualitative detection of porcine transmissible gastroenteritis virus by reverse transcription coupled real time polymerase chain reaction

 

Transmissible gastroenteritis (TGE) is an enteric disease of pigs. TGE virus (TGEV) is an RNA virus belonging to the genus Coronavirus of the family Coronaviridae. The virus is very stable when stored frozen, but labile at room temperature or above. It is also vulnerable to sunlight and oxidizing disinfectants such as bleach or iodine solution.

The major route of transmission of TGEV is fecal-oral. Once pigs are infected, the incubation period varies from 18 hours to 3 days. The virus multiplies in the intestines and damages enterocytes lining the small intestine, producing villous atrophy and enteritis. Major symptoms include diarrhea and vomiting. The mortality rate is high in neonates. Extra-intestinal sites of virus multiplication include the respiratory tract and mammary tissues, but the virus is most readily isolated from the intestinal tract and from feces.

It has been suggested that wild and domestic carnivores, such as foxes, dogs, and possibly mink and cats, serve as carriers of the virus and contribute to seasonal epidemics. However only virus excreted by serially TGEV-infected dogs has been confirmed as infectious for pigs.

Diagnosis by viral culture is time consuming and is not a very sensitive means of detection of this virus. Diagnosis by serological detection has the risk of cross reactivity with other similar viruses, such as porcine respiratory coronavirus. Molecular detection by PCR can provide rapid, sensitive and specific detection of the virus (Paton et al., 1997).

Utilities:

  • Help confirm the disease causing agent
  • Identify porcine TGEV carriers
  • Help ensure that animal groups and populations are free of porcine TGEV
  • Early prevention of spread of the virus among animals
  • Minimize human exposure to the virus
  • Safety monitoring of biological products that derive from animals

References:
P
aton, D., Ibata, G., Sands, J. and McGoldrick,  A. (1997) Detection of transmissible gastroenteritis virus by RT-PCR and differentiation from porcine respiratory coronavirus. J. Virol. Methods. 66:303-309.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml feces, or 0.2 ml fresh, frozen or fixed tissue, or nasal swab, or fecal swab.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

Porcine TGEV PCR test

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