We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

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Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Avian adenovirus

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Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine endogenous retrovirus

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Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

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Bovine viral diarrhea

Brachyspira pilosicoli

Brucella

Cache Valley virus

Campylobacter      

Candida

Caprine arthritis-encephalitis (CAE) virus

Chlamydophila psittaci

Classical swine fever

Clostridium

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Coxiella burnetii

Cryptococcus

Cryptosporidium

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Erysipelothrix rhusiopathiae

Foot and mouth disease

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Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma species

Mycoplasma suis

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Valley fever

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Vesicular exanthema of swine virus PCR test
avian & livestock assay data sheet

Vesicular exanthema of swine virus (VESV)

Test code: S0222 - Ultrasensitive detection of vesicular exanthema of swine virus by reverse transcription coupled real time PCR

Vesicular exanthema of swine virus (VESV) is a calicivirus. There are 13 serotypes of VESV and the virus is closely related to at least 14 other calicivirus serotypes found in the San Miguel sea lion virus (SMSV) group. Many SMSVs have been shown to cause vesicular disease when they are experimentally inoculated into pigs.

Low levels of antibodies to VESVs and SMSVs have been found in terrestrial mammals along the West coast of the US including wild boars, foxes, buffaloes, donkeys, and cattle. This suggests that the host range of these viruses may extend beyond pigs and sea lions. Human infection with these viruses has not been documented.

Infected pigs may develop fever, lameness, and vesicles followed by erosions in the mouth and on the snout, feet, and teats. The infection is easily confused with foot-and-mouth disease (FMD). However, lesions in VESV-infected pigs seem to be deeper, and granulation tissue commonly forms especially on the feet. Morbidity can reach almost 100%, but mortality is low. Transmission of the virus is mainly through direct contact, oronasal and lachrymal secretions, urine, feces, insemination, blood transfer, or feeding of raw or insufficiently cooked meat from infected animals.

Diagnosis of VESV infection by serological methods is difficult because of its close antigenicity to SMSV and other caliciviruses. Molecular detection by PCR is highly sensitive, specific and rapid, and is a useful alternative to traditional methods (Neill and Seal, 1995).

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of VESV infection
  • Help ensure that animal herds and populations are free of VESV
  • Early prevention of spread of this virus among animals
  • Minimize human exposure to this virus
  • Safety monitoring of biological products that derive from animals

References:
Neill, J.D. and Seal, B.S. (1995) Development of PCR primers for specific amplification of two distinct regions of the genomes of San Miguel sea-lion and vesicular exanthema of swine viruses. Mol. Cell Probes 9:33-37.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or oral, nasal or eye swab, or 0.2 ml feces, or rectal swab, or 0.2 ml urine, or 0.2 ml fresh or frozen tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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