We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

Parrots moving in or moving out? Try our psittacine PCR screening panel.

Respiratory problems got you breathless? Try our poultry respiratory PCR panel.

Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere because DRY CARD samples are small, light and stable at room temperature for several weeks.

Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea

Brachyspira pilosicoli

Brucella

Cache Valley virus

Campylobacter      

Candida

Caprine arthritis-encephalitis (CAE) virus

Chlamydophila psittaci

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Cowpox

Coxiella burnetii

Cryptococcus

Cryptosporidium

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Foot and mouth disease

Fowlpox

Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

avian & livestock assay data sheet

Borna virus

Test code:
S0207
- Ultrasensitive qualitative detection of Borna virus by reverse transcription coupled real time polymerase chain reaction

 

Borna disease virus (BDV) is an enveloped nonsegmented negative-strand RNA virus with a genome size of about 9 kb. The virus is of member of the Mononegavirales order. The Mononegavirales also include Filoviridae (eg Marburg and Ebola viruses), Paramyxoviridae (eg mumps, measles virus), and Rhabdoviridae (eg rabies, vesicular stomatitis virus).

This viral disease was first described more than 200 years ago in a small town named Borna in Saxony in southern Germany. It is a fatal neurologic disease of horses and sheep. A large number of horses died during an epidemic in 1885. Although outbreaks of Borna disease are rare, serological survey has indicated that many horses in various geographic regions have been exposed to the virus. This suggests that natural infection of horses with this virus may be subclinical.

Although horses are the natural host of the virus, other equidae, sheep, cattle, rabbits, goats, deer, alpacas, llamas, cats, pygmy hippopotamus, sloths and ostriches can be infected with BDV. The virus is transmitted by direct contact with saliva, nasal discharge or conjunctival secretions of infected animals.  Direct exposure to contaminated food or water can also be a source of infection.

Infected horses or sheep usually take about 4 weeks to show signs of infection, but the signs are non-specific. These signs include hyperthermia, anorexia, colic, and constipation in the initial phase of the disease. During the acute phase, neurologic signs such as ataxia, depression, circular movement, standing in awkward positions, collapsing, running into obstacles, and paralysis, may develop. Clinical symptoms last 1 to 3 weeks, and death rates for diseased horses are 80% to 100%.

Diagnosis of Borna disease can be by serological methods or by molecular methods such as polymerase chain reaction (PCR) method. PCR is rapid, sensitive and specific (Wensman et al., 2012), and does not require infected animals to develop full immune responses. Thus, PCR is especially suitable for early detection of the virus.

Utilities:

  • Help confirm the disease causing agent
  • Identify Borna virus carriers
  • Help ensure that animal herds and populations are free of Borna virus
  • Early prevention of spread of this virus among animals
  • Minimize human exposure to this virus
  • Safety monitoring of biological products that derive from animals

References:
Wensman, J.J., Jäderlund, K.H., Gustavsson, M.H., Hansson-Hamlin, H., Karlstam, E., Lilliehöök, I., Oström, I.L., Belák, S., Berg, M. and Holst, B.S. (2012) Markers of Borna disease virus infection in cats with staggering disease. J. Feline Med. Surg. 14:573-582.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or oral swabs, or nasal swabs, or 0.2 ml fresh or frozen tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

Borna virus PCR test

©2003-2016 Zoologix, Inc. • Email Zoologix • Phone (818) 717-8880