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Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

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Avian adenovirus

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Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

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Bovine ephemeral fever virus

Bovine herpesvirus 1

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Bovine herpesvirus 4

Bovine leukemia virus

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Bovine viral diarrhea

Brachyspira pilosicoli

Brucella

Cache Valley virus

Campylobacter      

Candida

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Chlamydophila psittaci

Classical swine fever

Clostridium

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Coronaviruses

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Coxiella burnetii

Cryptococcus

Cryptosporidium

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Erysipelothrix rhusiopathiae

Foot and mouth disease

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Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Japanese encephalitis PCR test for birds and livestock
avian & livestock assay data sheet

Japanese encephalitis

Test code:
S0062 - Ultrasensitive qualitative detection of Japanese encephalitis virus by reverse transcription coupled real time polymerase chain reaction

 

One of the leading causes of acute encephalopathy in children in the tropics is Japanese encephalitis (JE). An arbovirus, the Japanese encephalitis virus is transmitted by Culex mosquitoes and is a member of genus Flavivirus of the family Flaviviridae. The RNA genome of the JE virus is positive sense, single-stranded, approximately 11 Kb in length, and contains one long open reading frame.

JE virus is related to Murray Valley encephalitis virus. The virus is neurotropic and predominately affects the thalamus, anterior horns of the spinal cord, cerebral cortex, and cerebellum. It mainly affects children <15 years of age and is mostly asymptomatic. The occasional symptomatic child typically presents with a neurological syndrome characterized by altered sensorium, seizures, and features of intracranial hypertension. Though no antiviral drug is available against JE, effective supportive management can improve the outcome. Control of JE involves efficient vector control and appropriate use of vaccines.

Horses and primates can develop similar pathological lesions to those in human when infected with JE virus; they are considered dead-end hosts for JE. Most horses infected by JE virus show mild clinical signs, including fever, anorexia and depression. However, the mortality rate is high when JE infected horses show neurological symptoms (Ihara et al., 1997). Seroepidemiological survey of Asian monkeys has also shown widespread infection of these primates with JE virus (Yuwono et al., 1984). The virus can also infect birds, pigs and donkeys. Currently, the virus is mainly detected in East Asia, southeast Russia, India, Papua New Guinea and the Torres Strait Islands.

Conventional methods of JE diagnosis, including hemagglutination-inhibition and complement fixation tests for antibody assay, have been ineffective because of low sensitivity. Although a new immunoassay was developed to detect earlier, virus-specific IgM antibodies in the serum and cerebrospinal fluid (CSF) of acute and convalescent-phase patients, this new assay also suffered from low sensitivity and non-specific reaction. Reverse transcription-polymerase chain reaction (RT-PCR) has been used to detect Flavivirus rapidly and specifically.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of JE infection.
  • Help ensure that flocks are free of JE virus
  • Early prevention of spread of the virus
  • Minimize personnel exposure to the virus
  • Safety monitoring of biological products and vaccines that derive from birds

References:
Ihara, T., Kano, R., Nakajima, Y., Sugiura, T., Imagawa, H., Izuchi, T. and Samjima, T. (1997) Detection of antibody to Japanese encephalitis virus (JEV) by enzyme-linked immunosorbent assay (ELISA). J. Equine Sci. 8: 25-28.
Yuwono, J., Suharyono, W., Koiman, I., Tsuchiya, Y. and Tagaya, I. (1984) Seroepidemiological survey on dengue and Japanese encephalitis virus infections in Asian monkeys. Southeast Asian J Trop Med Public Health. 15:194-20

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml fresh or frozen CNS tissue, or 0.2 ml CSF, serum or plasma.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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