We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

Parrots moving in or moving out? Try our psittacine PCR screening panel.

Respiratory problems got you breathless? Try our poultry respiratory PCR panel.

Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere because DRY CARD samples are small, light and stable at room temperature for several weeks.

Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea

Brachyspira pilosicoli

Brucella

Cache Valley virus

Campylobacter      

Candida

Caprine arthritis-encephalitis (CAE) virus

Chlamydophila psittaci

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Cowpox

Coxiella burnetii

Cryptococcus

Cryptosporidium

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Foot and mouth disease

Fowlpox

Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Porcine cytomegalovirus PCR test
avian & livestock assay data sheet

Porcine enterovirus (PEV 8, 9 and 10)

Test code:
S0210 - Ultrasensitive detection but not differentiation of porcine enteroviruses (PEV 8, 9 and 10) by reverse transcription coupled real time PCR

 

Porcine enteroviruses are porcine picornaviruses and are ubiquitous in nature. Porcine enteroviruses (PEV) formerly comprised at least 13 serotypes, grouped into three species. However, porcine enterovirus 1 (PEV 1, the prototype “Teschen virus” and “Talfan virus”), PEV 2 through 7, and PEV 11-13 have now been moved to a new genus called Teschovirus. Porcine enteroviruses 8-10 remain in the original Enterovirus genus.

Infection with these viruses can sometimes cause neurological disorders, fertility disorders, and dermal lesions of swine. However, most infections are asymptomatic.

Infected pigs can shed the viruses in feces. Spread of the virus is usually by direct or indirect contact with infected pigs, and transmission is usually through the fecal-oral route. Infected fetuses born alive are sometimes a source of viral exposure of other pigs. Fomites can also carry the virus, which is relatively stable in the environment. All ages of swine are susceptible if they have not previously encountered a picornavirus of that serogroup.

Serological detection of infection requires paired serum samples taken at least two weeks apart to show a rising titer in serum neutralization or complement fixation tests. Virus isolation in tissue culture and identification by immunofluorescence or identification of viral antigen in tissue by similar technique may be useful. However, these techniques are not available in all diagnostic laboratories in the United States because virulent porcine picornaviral infections seldom occur there. Secondly, those techniques are not very sensitive and are relatively slow. Molecular detection by PCR, on the other hand, is increasingly the method of choice because of its sensitivity, specificity and faster turnaround time (La Rosa et al., 2006).

Utilities:

  • Help confirm the disease causing agent
  • Identify PEV carriers
  • Help ensure that facilities and populations are free of PEV
  • Early prevention of spread of the virus among animals
  • Minimize human exposure to the virus
  • Safety monitoring of biological products that derive from animals

References:
La Rosa, G., Muscillo, M., Di Grazia, A., Fontana, S., Iaconelli, M. and Tollis, M. (2006) Validation of rt-PCR assays for molecular characterization of porcine teschoviruses and enteroviruses. J. Vet. Med. B. Infect. Dis. Vet. Public Health 53:257-265.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml feces, or fecal or rectal swabs, or 0.2 ml urine, or 0.2 ml cerebrospinal fluid, or 0.2 ml fresh or frozen tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription real time PCR

Normal range: Nondetected

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