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Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

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Blood typing for swine

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Brachyspira pilosicoli

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Cache Valley virus

Campylobacter      

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Chlamydophila psittaci

Classical swine fever

Clostridium

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Enteric E. coli panel

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Fusobacterium necrophorum

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Mycobacterium avium and other Mycobacteria

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Ornithobacterium rhinotracheale

Ovine herpesvirus 2

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Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

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Porcine cytomegalovirus

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Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

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Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

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Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

avian & livestock assay data sheet

Cache Valley virus

Test code:
S0206
- Ultrasensitive qualitative detection of Cache Valley virus by reverse transcription coupled real time polymerase chain reaction

 

Cache Valley virus is a negative-sense, single-stranded RNA virus belonging to the Bunyamwera serogroup in the genus Orthobunyavirus and was named after the location in northern Utah where it was first isolated from mosquitoes. The virus can infect many species of domestic and wild animals such as deer, cattle, sheep, and horses, and is a concern in animal husbandry due to its ability to cause abortion, stillbirth, and congenital defects of the musculoskeletal and central nervous systems in affected ruminants. Recently, several cases of human infection have been reported.

The virus has been found throughout the U.S., Canada, and Mexico. There is no vaccine and there is no known treatment available. The most effective method of protecting animals from the Cache Valley virus is to minimize their exposure to mosquito-infested areas during and shortly after the breeding season.

Traditionally, diagnosis of this viral infection has been achieved by measuring the rising titer of antibody production in infected animals, but antibody production can be slow. Molecular detection by PCR is rapid, sensitive and specific (Wang et al., 2009), and is an important alternative to the traditional method of diagnosis.

Utilities:

  • Help confirm the disease causing agent
  • Identify Cache Valley virus carriers
  • Help ensure that animal groups and populations are free of Cache Valley virus
  • Early prevention of spread of this virus among animals
  • Minimize human exposure to this virus
  • Safety monitoring of biological products that derive from animals

References:
Wang, H., Nattanmai, S., Kramer, L.D., Bernard, K.A. and Tavakoli, N.P. (2009) A duplex real-time reverse transcriptase polymerase chain reaction assay for the detection of California serogroup and Cache Valley viruses. Diagn. Microbiol. Infect. Dis. 65:150-157.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml cerebrospinal fluid, or 0.2 ml fresh or frozen tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

Cache Valley virus PCR test

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