We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

Parrots moving in or moving out? Try our psittacine PCR screening panel.

Respiratory problems got you breathless? Try our poultry respiratory PCR panel.

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Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea

Brachyspira pilosicoli

Brucella

Cache Valley virus

Campylobacter      

Candida

Caprine arthritis-encephalitis (CAE) virus

Chlamydophila psittaci

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Cowpox

Coxiella burnetii

Cryptococcus

Cryptosporidium

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Erysipelothrix rhusiopathiae

Foot and mouth disease

Fowlpox

Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Vesicular stomatitis PCR test

avian & livestock assay data sheet

Vesicular stomatitis virus (VSV)

Test code: S0068 - Ultrasensitive qualitative detection of vesicular stomatitis virus by reverse transcription coupled real time polymerase chain reaction. This assay detects and differentiates the New Jersey and Indiana serotypes of VSV.

 

Vesicular stomatitis (VS) is a disease of horses, cattle, swine, sheep, goats and deer caused by related viruses in the genus Vesiculovirus of the family Rhabdoviridae. The viral genome is 11 kb and codes for five main proteins: the nucleocapsid (N), phosphoprotein (P), matrix (M), glycoprotein(G), and polymerase (L). Serologically, VS viruses have been classified into two main serotypes: New Jersey (NJ) and Indiana (IN). In addition to the classic VSV-IN (IN1), two additional subtypes, Cocal virus (VSV-IN2) and Alagoas virus (VSV-IN3), have been described.

VS is prevalent in northern South America, throughout Central America, and at one site in the United States: Ossabaw Island, Georgia. However, outbreaks of VS have been reported in the western United States, northern Mexico, and temperate regions of South America. The disease can cause blisters on an animal's mouth, teats or hooves. Clinical outbreaks of VS in livestock can result in significant economic impact on producers and extensive regulatory responses by governmental agencies, including trade restrictions, market closures, and quarantines. Such measures are needed because of the clinical similarities between VS and foot-and-mouth disease and other foreign vesicular diseases in livestock species.

Utilities:

  • Help confirm the disease causing agent
  • Distinguish between VS and other vesicular diseases including foot-and-mouth
  • Shorten the time required to confirm a clinical diagnosis of VS infection
  • Help ensure that animal herds and populations are free of VS
  • Early prevention of spread of VS
  • Minimize human exposure to the virus

References:
Magnuson, R.J., Triantis, J., Rodriguez, L.L., Perkins, A., Meredith, C.O., Beaty, B., McCluskey, B., and Salman M. (2003) A single-tube multiplex reverse transcription-polymerase chain reaction for detection and differentiation of vesicular stomatitis Indiana 1 and New Jersey viruses in insects. J. Vet. Diagn. Invest. 15:561-567.

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or lesion swab or nasopharyngeal swab, or 0.2 ml nasal wash, or 0.2 ml fresh or frozen tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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