avian & livestock assay data sheet
bronchitis virus (IBV)
Ultrasensitive qualitative detection of infectious bronchitis
virus by reverse transcription coupled real time polymerase
chain reaction. This assay detects but does not
differentiate the Connecticut and Massachusetts strains of
S0086 is included in the
poultry respiratory panel
Infectious bronchitis in
chickens is caused by avian infectious bronchitis virus (IBV), a
coronavirus. Infected chickens develop various respiratory signs
including gasping, coughing, sneezing, tracheal rales, and nasal
discharge. In young chickens, severe respiratory distress may
Besides attacking the respiratory tract of infected chickens,
the virus can infect the uro-genital tract and other organs. In
addition to respiratory symptoms, infected birds may develop egg
drops or kidney damage.
Infectious bronchitis can be
difficult to diagnose by traditional means because symptoms are
similar to those of other poultry respiratory infections such as
Newcastle disease virus, metapneumovirus, and infectious
Although morbidity is high, the mortality rate in an IBV
infected flock varies depending on standards of management and
on possible involvement of secondary bacterial infections.
Traditionally IBV diagnosis relied on virus isolation, but this
method is not very sensitive. Serological diagnosis relies on
measuring antibody titers and may not be quick enough to allow
effective control of the spread of the disease. Molecular
detection by polymerase chain reaction (PCR) enables rapid,
sensitive, and specific identification of the virus (Zwaagstra
et al., 1992).
Help confirm the disease causing agent
Help ensure that bird populations are free of IBV
Early prevention of spread of the virus among bird
Minimize human exposure to the virus
Safety monitoring of biological products and vaccines that
derive from birds
Zwaagstra, K.A., van der Zeijst, B.A.M. and Kusters, J.G.
(1992). Rapid detection and identification of avian bronchitis
virus. J. Clin. Microbiol., 30, 79–84.
Specimen requirements: Tracheal swab.
For specimen types other than those listed here, please call to
confirm specimen acceptability and shipping instructions.
For all specimen types, if there will be a delay in shipping, or
during very warm weather, refrigerate specimens until shipped
and ship with a cold pack unless more stringent shipping
requirements are specified. Frozen specimens should be shipped
so as to remain frozen in transit. See
shipping instructions for
Turnaround time: 2 business days
Methodology: Qualitative reverse
transcription coupled real time PCR
Normal range: Nondetected