We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

Parrots moving in or moving out? Try our psittacine PCR screening panel.

Respiratory problems got you breathless? Try our poultry respiratory PCR panel.

Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere because DRY CARD samples are small, light and stable at room temperature for several weeks.

Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea

Brachyspira pilosicoli

Brucella

Cache Valley virus

Campylobacter      

Candida

Caprine arthritis-encephalitis (CAE) virus

Chlamydophila psittaci

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Cowpox

Coxiella burnetii

Cryptococcus

Cryptosporidium

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Foot and mouth disease

Fowlpox

Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Infectious bronchitis PCR test for poultry
avian & livestock assay data sheet

Infectious bronchitis virus (IBV)

Test code:
S0086
- Ultrasensitive qualitative detection of infectious bronchitis virus by reverse transcription coupled real time polymerase chain reaction.  This assay detects but does not differentiate the Connecticut and Massachusetts strains of IBV.

S0086 is included in the poultry respiratory panel

Infectious bronchitis in chickens is caused by avian infectious bronchitis virus (IBV), a coronavirus. Infected chickens develop various respiratory signs including gasping, coughing, sneezing, tracheal rales, and nasal discharge. In young chickens, severe respiratory distress may occur.

Besides attacking the respiratory tract of infected chickens, the virus can infect the uro-genital tract and other organs. In addition to respiratory symptoms, infected birds may develop egg drops or kidney damage.

Infectious bronchitis can be difficult to diagnose by traditional means because symptoms are similar to those of other poultry respiratory infections such as Mycoplasma gallisepticum, Newcastle disease virus, metapneumovirus, and infectious laryngotracheitis virus.

Although morbidity is high, the mortality rate in an IBV infected flock varies depending on standards of management and on possible involvement of secondary bacterial infections.

Traditionally IBV diagnosis relied on virus isolation, but this method is not very sensitive. Serological diagnosis relies on measuring antibody titers and may not be quick enough to allow effective control of the spread of the disease. Molecular detection by polymerase chain reaction (PCR) enables rapid, sensitive, and specific identification of the virus (Zwaagstra et al., 1992).

Utilities:

  • Help confirm the disease causing agent
  • Environmental monitoring
  • Help ensure that bird populations are free of IBV
  • Early prevention of spread of the virus among bird populations
  • Minimize human exposure to the virus
  • Safety monitoring of biological products and vaccines that derive from birds

References:
Zwaagstra, K.A., van der Zeijst, B.A.M. and Kusters, J.G. (1992). Rapid detection and identification of avian bronchitis virus. J. Clin. Microbiol., 30, 79–84.

Specimen requirements: Tracheal swab.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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