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Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Avian adenovirus

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Blood typing for swine

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Bordetella avium

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Bovine viral diarrhea

Brachyspira pilosicoli

Brucella

Cache Valley virus

Campylobacter      

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Chlamydophila psittaci

Classical swine fever

Clostridium

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Coxiella burnetii

Cryptococcus

Cryptosporidium

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Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Erysipelothrix rhusiopathiae

Foot and mouth disease

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Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

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Infectious coryza

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Influenza

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma species

Mycoplasma suis

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Valley fever

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Cryptosporidium PCR test for birds
avian & livestock assay data sheet

Cryptosporidium

Test codes:

P0008 - Ultrasensitive screen for Cryptosporidium by real time polymerase chain reaction. This screen detects but does not differentiate Cryptosporidium species, utilizing a real time PCR procedure to achieve highly sensitive detection.

Test P0008 is included in P0041 - waterborne pathogens screening panel

                                                                     

Several of the 13+ species in the Cryptosporidium genus have been confirmed as causative agents of human disease. Cryptosporidium is a parasitic protozoan that is transmitted by multiple routes; the animal host range is diverse. The following Cryptosporidium species are currently accepted, on the basis of host specificity, pathogenesis, morphology and genotyping:

Infecting mammals: Cryptosporidium hominis, C. parvum, C. wrairi, C. felis, C. canis, C. andersoni, C. muris and C. ubiquitum

Infecting birds: C. baileyi, C. meleagridis and C. galli

Infecting reptiles: C. serpentis and C. saurophilum

Infecting fish: C. molnari

Phylogenetic analyses have been largely based on sequencing of the small subunit rRNA gene (18S rRNA), the hsp 70 gene, or other housekeeping or structural genes. These analyses reveal that the various Cryptosporidium species interact in complex ways with hosts. For example, the specific host of C. felis is cats, but this species has also been isolated from a cow, while C. andersoni is morphologically close to C. muris but infects cattle rather than mice. And C. parvum includes a complex of subspecies that specifically infect cattle, pigs, kangaroos, ferrets or monkeys.

With the advance of molecular techniques, knowledge of the epidemiology of human cryptosporidiosis has significantly increased. It has been shown that the vast majority of human cases are caused by C. hominis (synonymous with C. parvum genotype 1) and C. parvum (synonymous with C. parvum genotype 2). Other species, including C. meleagridis, C. felis, C. canis and C. muris can also infect humans and are linked to clinical disease, not only in immunocompromised patients but also in immunocompetent people.

PCR is a rapid and extremely sensitive technique for detection of Cryptosporidium.

Utilities:

  • Help confirm the disease causing agent
  • Shorten the time required to confirm a clinical diagnosis of Cryptosporidium infection.
  • Help ensure that bird flocks are free of Cryptosporidium
  • Early prevention of spread of this protozoan
  • Minimize human exposure to this protozoan

References:
Thomas, A.L. and Chalmers, R.M. (2003) Investigation of the range of Cryptosporidium species detected by commercially available antibody-based tests. Proceedings of the Health Protection Agency Inaugural Conference, Warwick, September.

Specimen requirements: Rectal swab, or cloacal swab, or 0.2 ml feces.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative real time PCR

Normal range: Nondetected

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