We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

Parrots moving in or moving out? Try our psittacine PCR screening panel.

Respiratory problems got you breathless? Try our poultry respiratory PCR panel.

Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere because DRY CARD samples are small, light and stable at room temperature for several weeks.

Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine endogenous retrovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea

Brachyspira pilosicoli

Brucella

Cache Valley virus

Campylobacter      

Candida

Caprine arthritis-encephalitis (CAE) virus

Chlamydophila psittaci

Classical swine fever

Clostridium

Coccidia

Coccidiodes

Coronaviruses

Cowpox

Coxiella burnetii

Cryptococcus

Cryptosporidium

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Erysipelothrix rhusiopathiae

Foot and mouth disease

Fowlpox

Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma species

Mycoplasma suis

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Valley fever

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Avian and mammalian reovirus PCR screens

avian & livestock assay data sheet

Reoviruses

Test codes:

S0083 - Ultrasensitive qualitative screen for avian reoviruses by reverse transcription coupled real time PCR. This assay detects but does not differentiate most common strains of avian reovirus, including S1133, 1733, 2408 and CO8.

S0120 - Ultrasensitive qualitative screen for mammalian reoviruses by reverse transcription coupled real time PCR.  This assay detects a wide range of mammalian reoviruses but does not detect avian reovirus strains.

 

Reoviruses are comprised of 10 to 12 double-stranded RNA genomic segments that can reassort both in nature and in laboratory settings. The most common mammalian isolates are type 1 (Lang), type 2 (Jones), and type 3 (Dearing).

Reoviruses have a high endemic infection rate in many mammals, such as primates, cattle, cats, dogs, rodents and swine. These viruses are common in raw water sources and are often found along with other animal viruses. In humans, the viruses cause only asymptomatic or mild respiratory infections. However, research suggests that reoviruses may be associated with potentially more severe illnesses. Reoviruses have been linked to neonatal hepatitis, extrahepatic biliary atresia, meningitis and myocarditis. Also, immunocompromised, young and elderly individuals may become susceptible to severe bacterial respiratory disease due to an initial reovirus infection.

Due to their widespread occurrence and the ability of these viruses to survive a long period of time in the environment, contamination of water sources has been frequently reported. Animals are especially prone to infection by these viruses. Xenotransplantation of animal organs is severely endangered by potential contamination with these viruses.

Diagnosis of reovirus infection by nonmolecular means is very difficult and is usually based on virus isolation on cell cultures and electron microscopy. Those methods are not very sensitive (Muscillo et al., 2001) and are likely to underestimate the presence of these viruses in animals and humans. Molecular detection by PCR is the most sensitive, rapid and specific method for identifying reoviruses.

Utilities:

  • Help confirm the disease causing agent
  • Help ensure that animal groups and populations are free of reoviruses
  • Early prevention of spread of reoviruses among a population
  • Minimize human exposure to reoviruses
  • Safety monitoring of biological products and vaccines that derive from susceptible animals

References:
Muscillo M., La Rosa G., Marianelli C., Zaniratti S., Capobianchi M.R., Cantiani L. and Carducci A. (2001) A new RT-PCR method for the identification of reoviruses in seawater samples, Water Res. 35:548–556.

Specimen requirements: Tracheal swab, nasal swab, cloacal swab or rectal swab, or 0.2 ml feces, or 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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