We've added new PCR tests for swine and bovine diseases -- see our menu for a complete listing.

Parrots moving in or moving out? Try our psittacine PCR screening panel.

Respiratory problems got you breathless? Try our poultry respiratory PCR panel.

Our DRY CARDS let you mail blood samples to Zoologix easily and cheaply from anywhere because DRY CARD samples are small, light and stable at room temperature for several weeks.

Zoologix performs avian and livestock PCR tests for...

Actinobacillus pleuropneumoniae

African swine fever

Akabane virus

Alcelaphine herpesvirus

Anaplasma phagocytophilum

Aspergillus fumigatus

Aspergillus species

Atoxoplasma

Avian adenovirus

Avian herpes

Avian influenza

Avian polyomavirus

Avian reovirus

Baylisascaris procyonis

Blood typing for swine

Bluetongue virus

Bordetella avium

Borna virus

Bovine adenovirus

Bovine enterovirus

Bovine ephemeral fever virus

Bovine herpesvirus 1

Bovine herpesvirus 2

Bovine herpesvirus 4

Bovine leukemia virus

Bovine papillomavirus

Bovine papular stomatitis virus

Bovine parvovirus

Bovine polyomavirus

Bovine respiratory syncytial virus

Bovine rhinoviruses

Bovine viral diarrhea

Brachyspira pilosicoli

Brucella

Cache Valley virus

Campylobacter      

Candida

Caprine arthritis-encephalitis (CAE) virus

Chlamydophila psittaci

Classical swine fever

Clostridium

Coccidia

Coronaviruses

Cowpox

Coxiella burnetii

Cryptococcus

Cryptosporidium

E. coli O157:h7

Edwardsiella

Encephalomyocarditis

Enteric E. coli panel

Foot and mouth disease

Fowlpox

Fusobacterium necrophorum

Hepatitis E

Herpes, avian

Histoplasma

Infectious bronchitis

Infectious bursal disease

Infectious coryza

Infectious laryngotracheitis

Influenza

Jaagsiekte sheep retrovirus (JSRV)

Japanese encephalitis

Jena virus

Johne's disease

Leptospira

Lumpy skin disease virus

Malaria

Malignant catarrhal fever (MCF)

Mites

Mycobacterium avium and other Mycobacteria

Mycoplasma

Newcastle disease virus

Nipah virus

Ornithobacterium rhinotracheale

Ovine herpesvirus 2

Pacheco's disease (psittacid herpesviruses)

Peste des petits ruminants virus (PPRV)

Pigeon circovirus

Plasmodium species

Porcine adenovirus

Porcine circovirus 1

Porcine circovirus 2

Porcine cytomegalovirus

Porcine endogenous retrovirus (PERV)

Porcine enterovirus

Porcine epidemic diarrhea virus

Porcine hemagglutinating encephalomyelitis

Porcine lymphotropic herpesvirus

Porcine parvovirus

Porcine reproductive & respiratory syndrome (PRRS) virus

Porcine respiratory coronavirus (PRCV)

Porcine transmissible gastroenteritis virus (TGEV)

Poultry respiratory panel

Pseudocowpox

Pseudorabies

Psittacine beak and feather disease

Psittacine herpes

Q fever

Rabies

Reovirus

Rift Valley fever virus

Rinderpest virus

Salmonella

Staphylococcus xylosus

St. Louis encephalitis

Streptococcus

Swinepox

Swine vesicular disease

Teschovirus (Teschen-Talfan disease)

Tickborne encephalitis virus

Trichomonas/
Tritrichomonas

Vaccinia

Vesicular exanthema of swine

Vesicular stomatitis

Wesselsbron virus

West Nile virus

Yersinia enterocolitica

Yersinia pseudotuberculosis

...and more -- see the avian & livestock test menu for a complete listing of avian and livestock assays.

Bovine respiratory syncytial virus PCR test
avian & livestock assay data sheet

Bovine respiratory syncytial virus (BRSV)

Test code: S0155
- Ultrasensitive detection of bovine respiratory syncytial virus by reverse transcription coupled real time PCR.

Bovine respiratory syncytial virus (BRSV) is an RNA virus belonging to the paramyxoviridiae family. This virus is named for its characteristic cytopathic effect on cell culture, the formation of syncytial cells.

Initial exposure to the virus can result in severe respiratory disease, but subsequent exposures will only result in mild to subclinical disease due to developed immunity. Symptoms associated with the viral infection include high fever (104-108F), depression, decreased feed intake, increased respiratory rate, cough, and nasal and lacrimal discharge. Dyspnea, possibly with open-mouthed breathing, may occur in the later stages of the disease. Complications include secondary bacterial pneumonia. Lesions in the respiratory tract may occur.

BRSV is difficult to detect by culture, and the chances of isolation only improve when sampling animals that are in the incubation or acute phases of infection. Serological detection may be used, but paired samples are required, and this may take several weeks to accomplish. Molecular detection by PCR can provide highly specific, rapid and sensitive detection of the virus.

Utilities:            

  • Help confirm the disease causing agent
  • Identify BRSV carriers
  • Screen research materials for the presence of BRSV
  • Help ensure that animal groups are free of BRSV
  • Early prevention of spread of the virus among animals
  • Minimize human exposure to the virus
  • Safety monitoring of biological products that derive from susceptible animals

References:
Thonur, L., Maley, M., Gilray, J., Crook, T., Laming, E., Turnbull, D., Nath, M. and Willoughby, K. (2012)
One-step multiplex real time RT-PCR for the detection of bovine respiratory syncytial virus, bovine herpesvirus 1 and bovine parainfluenza virus 3. BMC Vet. Res. 8:37-45

Specimen requirements: 0.2 ml whole blood in EDTA (purple top) or ACD (yellow top) tube, or 0.2 ml cell culture, or swab, or tissue.

For specimen types other than those listed here, please call to confirm specimen acceptability and shipping instructions.

For all specimen types, if there will be a delay in shipping, or during very warm weather, refrigerate specimens until shipped and ship with a cold pack unless more stringent shipping requirements are specified. Frozen specimens should be shipped so as to remain frozen in transit. See shipping instructions for more information.

Turnaround time: 2 business days

Methodology: Qualitative reverse transcription coupled real time PCR

Normal range: Nondetected

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